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Best Probiotics: A Research-Based
Guide to Finding the Right Fit

PIM_ PIM_Gut Health_ Best Probiotics

Introduction

Probiotic shopping can become confusing fast. One label lists a single strain, another lists a dozen, and the CFU numbers can make it seem as though more must be better. Add broad “gut health” claims, and it becomes difficult to tell which differences actually matter.

We built this guide to make that comparison easier. Rather than naming one probiotic as the right choice for everyone, we explain how strain identity, formulation, CFU count, and individual context shape the evidence. Then we compare a focused group of products so you can narrow the options with a clearer sense of what may fit—and where the research still leaves uncertainty.

Purple Iris Media is an evidence-informed wellness publisher, not a medical provider. This article is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

This guide explains how probiotic products differ and how their labels can be compared with the available research. It does not determine whether a particular product is appropriate for you.

If you are immunocompromised, critically ill, managing a health condition, or unsure how a probiotic fits with your care, a qualified healthcare professional can help you consider the decision in your individual context.

What "Best" Means in This Guide

When we use the word “best,” we do not mean that one probiotic is right for everyone—or that products not included here are inferior. We use it to describe a focused group of products that met our review standards and represent different formulations, needs, and practical considerations.

These options were selected from a larger group reviewed before this guide was written. The goal is not to rank the entire market from first to last, but to give you a manageable set of well-vetted examples so the differences and trade-offs are easier to compare.

What Are Probiotics?

Probiotics are live microorganisms that, when consumed in adequate amounts, may support certain aspects of health [1][2]. Most probiotic products contain bacteria—often from the Lactobacillus or Bifidobacterium groups—although some use beneficial yeasts.

The important point is that probiotics are not interchangeable. Findings involving one strain, amount, formulation, or study population cannot automatically be applied to another product [3][5].

Why Strain Identity Matters

Two strains from the same species can behave differently. When a product identifies the complete strain, it is easier to compare that formula with published research. When strain information is missing, connecting the product to specific findings becomes much more difficult [3][5].

Why More CFUs Are Not Always Better

CFU, or colony-forming unit, counts describe the number of viable microorganisms in a product. Effective amounts depend on the strain and research context, so a higher count alone does not show that one product is better or more likely to help. Strain relevance, formulation, storage, and individual tolerance also matter [5][6][7].

Bacterial and Yeast Probiotics Are Different

Most probiotics are bacterial, but products containing Saccharomyces boulardii use a beneficial yeast. Yeast probiotics are not affected by antibacterial medications in the same way as bacterial strains, but that does not make either type universally preferable [8][9].

People can respond differently to the same probiotic. Diet, health history, recent antibiotic use, and the existing mix of microorganisms in the gut may all play a role [4][10]. That is why we look closely at the specific organism, group of people, and outcome studied rather than assuming that one finding applies to every probiotic—or every person.

How We Vetted These Probiotics

We reviewed a larger group of probiotic supplements before selecting the products featured in this guide. Our evaluation focused on four areas that help us understand what a formula offers, where its evidence is strongest, and which trade-offs may matter in everyday use.

1. Strain Identity and Transparency

We looked for clearly identified probiotic organisms, including complete strain designations when available. This makes it easier to compare a formula with published research. When a product used a proprietary blend or provided limited strain information, we treated that as a trade-off rather than assuming details that were not disclosed.

2. Research Alignment

We considered whether the named strains, amounts, formulation, and intended use could be meaningfully connected to relevant human research. Evidence involving one strain or study population cannot automatically be applied to another product, and a higher CFU count alone does not establish greater effectiveness [5][6]. Research alignment can help place a formula in context, but it does not guarantee an individual result.

3. Product Quality and Verification

We reviewed current product labels and manufacturer information for ingredient identity, potency statements, storage instructions, and quality or testing claims. Third-party testing is noted only when we can connect the claim to the specific product. The absence of a certification does not by itself establish poor quality, but clear and verifiable information makes a product easier to evaluate.

4. Formulation and Practical Fit

We considered serving format, storage requirements, disclosed additives or allergens, and other details that may affect everyday use. We also considered cost in relation to the formula and typical use, but we did not treat price as proof of quality. Features such as delayed-release capsules were recorded as product characteristics—not assumed to provide a meaningful advantage unless evidence supported that conclusion.

Probiotic Supplement Reviews

The five products below are not ranked from best to worst. Each represents a different formulation approach, and our goal is to make those differences easier to compare—including strain transparency, storage, serving format, and practical trade-offs—without treating any option as universally better.

1. Align 24/7 Digestive Support

A Clearly Identified Single-Strain Formula

Align 24/7 Digestive Support contains one probiotic strain, Bifidobacterium longum 35624. This strain has been studied in adults with IBS and in adults experiencing recurring abdominal discomfort and bloating. Results have differed by dose and study population, so the research helps us understand the strain but should not be treated as a promise that the product will relieve a particular symptom [12][13].

Key Specifications

Formula Type

Single-strain probiotic

Probiotic Strain

Bifidobacterium longum 35624™

Labeled Potency

1 billion CFU at manufacture; at least 10 million CFU through the best-by date

Serving Size

1 capsule

Servings per Container

28

Product Form

Capsule

Contains Milk Ingredients

Yes

Dietary and Storage Information

Gluten-free; soy-free; vegetarian; no refrigeration required

Third-Party Tested

Not confirmed
The reviewed product information does not identify independent finished-product testing.

Why this might work for you:

  • The clearly identified B. longum 35624 strain makes it easier to compare the formula with strain-specific human research.
  • The single-strain formulation provides a simpler alternative to products containing several organisms without assuming that more strains are necessarily better.
  • The labeled serving is one capsule per day, and the product does not require refrigeration.

Things to consider:

  • Research involving strain 35624 has produced different findings across doses and study populations, so the results should not be treated as a prediction of individual symptom relief [12][13].
  • The label states 1 billion CFU at manufacture and at least 10 million CFU through the best-by date. The potency therefore should not be understood as one fixed amount throughout the product’s shelf life.
  • The product contains milk ingredients.

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2. Jarrow Formulas Jarro-Dophilus EPS, 25 Billion CFU

An Eight-Strain, Shelf-Stable Formula

Jarro-Dophilus EPS combines eight identified probiotic strains in a proprietary blend providing 25 billion CFU per capsule. The multi-strain formulation offers a different approach from a single-strain product, although the label does not disclose how much of each strain is included. Its enteric-coated capsule, individual blister packaging, and room-temperature storage are practical product features rather than evidence that it will produce a particular health outcome.

Key Specifications

Formula Type

Proprietary eight-strain probiotic blend

Probiotic Strains

Lacticaseibacillus rhamnosus R0011; Lactobacillus helveticus R0052; Pediococcus acidilactici R1001; Lacticaseibacillus casei R0215; Bifidobacterium longum BB536; Lactiplantibacillus plantarum R1012; Bifidobacterium breve R0070; Lactococcus lactis subsp. lactis R1058

Labeled Potency

25 billion CFU per serving; individual strain amounts are not disclosed

Serving Size

1 capsule

Servings per Container

60

Product Form

Enteric-coated veggie capsule; individually blister-packed

Contains Milk Ingredients

Yes, in trace amounts

Dietary and Storage Information

Gluten-free; vegetarian; iGen™ Non-GMO; no refrigeration required. Store in a cool, dry place below 77°F (25°C).

Third-Party Tested

Not confirmed
The reviewed product information does not identify independent finished-product testing.

Why this might work for you:

  • The label identifies all eight strains, making the formula more transparent than a blend that lists only species or genera.
  • One capsule provides the complete labeled serving of 25 billion CFU.
  • Individual blister packaging and room-temperature storage may be convenient when refrigeration is impractical.

Things to consider:

  • Because individual strain amounts are not disclosed, it is difficult to compare the complete formula with strain- and dose-specific research; findings involving one strain should not be assumed to apply to the full eight-strain blend [5].
  • The enteric coating is a formulation feature, but it should not be treated as proof of better outcomes for this product.
  • The formula contains trace amounts of milk and soy.

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3. Vital Nutrients Hyperbiotics Pro-15 Advanced Probiotic

A 15-Species Blend With Kiwifruit Powder

Hyperbiotics Pro-15 Advanced combines a proprietary blend of 15 probiotic species with 250 mg of Actazin® kiwifruit powder. The label provides the total blend amount and CFU count but does not identify individual strains or disclose how much of each organism is included. This makes the formula easy to describe broadly but difficult to connect with strain- and dose-specific research.

Key Specifications

Formula Type

Proprietary multi-species probiotic and kiwifruit powder formula

Probiotic Organisms

L. plantarum, L. fermentum, L. acidophilus, B. infantis, L. casei, B. longum, L. rhamnosus, B. lactis, L. reuteri, L. salivarius, L. paracasei, L. gasseri, B. bifidum, B. breve, and S. thermophilus. Strain-level identifiers are not disclosed.

Labeled Potency

15 billion CFU at manufacture; minimum 5 billion CFU at expiration. Individual organism amounts are not disclosed.

Actazin® Kiwifruit Powder per Serving

250 mg

Serving Size

1 tablet

Servings per Container

60

Product Form

Time-release vegan tablet

Dietary and Storage Information

Vegan; non-GMO; free from gluten, dairy, peanuts, tree nuts, fish, shellfish, soy, sesame, and egg; no refrigeration required

Why this might work for you:

  • The formula combines 15 probiotic species with a separately identified amount of kiwifruit powder.
  • The label states both the CFU count at manufacture and the minimum guaranteed at expiration.
  • The labeled serving is one tablet per day, and the product does not require refrigeration.

Things to consider:

  • The label does not provide strain-level identifiers or individual organism amounts, limiting comparison with strain- and dose-specific research [5].
  • The manufacturer’s survivability comparison is based on a model system rather than demonstrated superiority in human health outcomes.
  • The product contains kiwifruit powder, which may matter if you avoid kiwi or have previously reacted to it.

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4. Florastor Dual Action Probiotic Supplement

A Single-Strain Yeast Probiotic

Florastor contains Saccharomyces boulardii CNCM I-745, a probiotic yeast rather than a bacterial strain. Because antibacterial medicines target bacteria, this yeast is not directly affected in the same way. The strain has been studied in research involving antibiotic-associated diarrhea, but that evidence should not be treated as a recommendation to combine the product with an antibiotic without considering individual health circumstances [8][9].

Key Specifications

Formula Type

Single-strain yeast probiotic

Probiotic Strain

Saccharomyces boulardii CNCM I-745®

Amount per Serving

500 mg

Serving Size

2 capsules

Servings per Container

50

Product Form

Vegetarian capsule

Contains Lactose

Yes, derived from milk

Dietary and Storage Information

Gluten-free; vegetarian; non-GMO; store at room temperature; do not refrigerate

Why this might work for you:

  • The complete strain designation makes the product easier to compare with research involving S. boulardii CNCM I-745.
  • Its yeast-based formulation provides an alternative to products containing bacterial organisms.
  • The capsules are stored at room temperature and do not require refrigeration.

Things to consider:

  • Research involving antibiotic-associated diarrhea does not mean that the product is appropriate for every person taking an antibiotic. A pharmacist or other qualified healthcare professional can help evaluate that decision [8][9].
  • The product contains lactose derived from milk, although the manufacturer describes it as appropriate for people with lactose intolerance.
  • Probiotic yeast generally warrants additional caution for people who are critically ill, severely immunocompromised, or have a central venous catheter because rare bloodstream infections have been reported [8].

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5. Garden of Life RAW Probiotics Ultimate Care Shelf-Stable

A 34-Organism Formula With a Once-Daily Serving

Some people prefer the simplicity of a single-strain probiotic. Others may be looking for a formula that brings several types of organisms together in one capsule. Garden of Life RAW Probiotics Ultimate Care takes the second approach, combining four identified strains with a larger kefir-derived blend, protein-digesting enzymes, and a fruit and vegetable blend.

The label provides the total potency but not the amount of each organism. It is also helpful to treat “Ultimate Care” and “Replenish Blend” as product names, not as evidence that the complete formula will restore or repopulate anyone’s gut microbiome.

Key Specifications

Formula Type

Proprietary multi-organism probiotic formula with protein-digesting enzymes and a fruit and vegetable blend

Identified Probiotic Strains

Bifidobacterium lactis BL818; Lactobacillus acidophilus SD-5221; Lactobacillus paracasei SD-5218; Bifidobacterium lactis SD-5220

Additional Probiotic Organisms

30 bacterial and yeast organisms and cultures are listed without strain identifiers; individual amounts are not disclosed

Labeled Potency

100 billion CFU through expiration under recommended storage conditions; individual organism amounts are not disclosed

Protein-Digesting Enzyme Blend per Serving

50 mg—protease, acid protease, and protease S

Fruit and Vegetable Blend per Serving

45 mg—red bell pepper, green pea, carrot, plum, cherry, strawberry, and raspberry

Serving Size

1 capsule

Servings per Container

30

Product Form

Vegetarian capsule

Contains Milk Ingredients

Yes

Dietary and Storage Information

Non-GMO Project Verified; NSF Certified Gluten-Free; shelf-stable; no refrigeration required; store in a cool, dry place

Third-Party Tested

Confirmed
The exact product displays Non-GMO Project Verified and NSF Certified Gluten-Free certifications. This confirmation applies only to those certified attributes and does not establish broader finished-product potency or purity testing.

Why this might work for you:

  • One capsule provides the complete labeled daily serving.
  • Four organisms include strain identifiers, offering more detail than a formula that lists species alone.
  • The labeled 100 billion CFU applies through expiration when the product is stored as directed.
  • The shelf-stable format does not require refrigeration.

Things to consider:

  • Label transparency: Most organisms lack strain identifiers and individual amounts.
  • Evidence interpretation: A large CFU count, long organism list, or research on one strain does not establish the effects of the complete formula.
  • Formula complexity: With probiotics, enzymes, and a fruit-and-vegetable blend, a personal response may be difficult to attribute to one component.
  • Ingredient suitability: The product contains milk.

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How to Choose the Right Probiotic for Your Needs

Probiotic labels can make it seem as though the product with the highest CFU count or the longest organism list must be the stronger choice. Those numbers tell us something about the formula, but they do not tell us whether it matches a particular question, body, or routine.

A more useful approach is to look at what the label identifies, how closely the formula can be connected with research, and whether the practical details work for you.

Start With the Question You Want to Answer

Some probiotic research examines a particular strain in a particular context. If you are exploring a probiotic for a specific health concern, look for evidence involving the exact strain or formulation rather than relying on a broad digestive-support claim.

If you are comparing products more generally, you can still prioritize transparent labeling and practical fit without assuming that a broader formula will necessarily be more useful.

Look at What the Label Actually Identifies

A complete strain designation includes the organism’s genus, species, and strain code. Some products provide this information for every organism. Others list species without strain identifiers or place several organisms in a proprietary blend.

A less detailed label does not automatically mean that the product is unsuitable. It does limit how closely you can compare the formula with strain-specific research, especially when individual amounts are not disclosed. [5]

Compare the Formula, Not Just the Headline Numbers

Higher CFU counts and longer organism lists do not reliably predict better results. Single-strain and multi-organism products represent different formulation approaches; neither is automatically preferable.

It is also worth noticing whether the product includes enzymes, prebiotics, plant ingredients, or other added components. These additions may affect practical fit, but research involving one component does not establish the effects of the complete formula.

Check the Practical Details

Serving size, capsule or tablet format, storage requirements, allergens, and verified dietary certifications can all affect whether a product fits your routine.

Review the complete label if you avoid particular ingredients or have food allergies or sensitivities. Convenience can make a product easier to use consistently, but it does not establish that the product will be more effective.

You do not need to solve every probiotic question at once. The goal is to make a choice whose trade-offs you understand: what the formula contains, how transparent the label is, how closely it relates to the available evidence, and whether it fits comfortably into your routine.

How to Take Probiotics

Once you have chosen a probiotic, the next question is often when and how to take it. Unfortunately, there is no single schedule that fits every product. Strains, serving sizes, storage requirements, and study conditions differ, so guidance for one probiotic should not automatically be applied to another.

Start With the Exact Product Directions

Follow the labeled serving, timing, and storage directions for the product you choose. Some labels allow use with or without food, while others provide more specific instructions.

Avoid transferring a dose or schedule from a study—or from another probiotic—to a different formula. Research involving one strain, amount, or combination does not establish how another product should be used.

Keep the Formula as the Reference Point

If you are using a probiotic because of research you have read, check whether the product contains the same strain or formulation examined in that research. A matching species name without the same strain identifier is not necessarily equivalent.

Taking more than the labeled serving does not make a probiotic more likely to help. Higher CFU exposure is not automatically better, particularly when the additional amount has not been evaluated for the same purpose.

Keep Expectations Proportionate

Responses to probiotics vary. Even when a product appears useful, that does not necessarily mean its organisms have permanently become part of the gut microbiome. Research suggests that colonization and response can differ considerably from one person to another. [10]

It can help to begin with a clear reason for trying the product and realistic expectations about what you hope to notice. That gives you a more useful way to evaluate your experience than relying on CFU numbers or broad microbiome claims.

Know When Individual Guidance Matters

Probiotics have a history of apparently safe use in healthy people, but detailed safety information remains limited. NCCIH notes that the risk of harmful effects is greater for people with severe illnesses or compromised immune systems. [14]

A healthcare professional or pharmacist can help you evaluate the exact product if you have a serious underlying condition, take medications, are pregnant or nursing, or are considering probiotic use alongside antibiotics. This is especially important when the goal involves a diagnosed condition rather than general product comparison.

Common Questions About: Probiotics

We begin with formulation transparency: whether the label identifies the organisms clearly, provides meaningful potency information, and makes it possible to compare the product with relevant research.

We also look for products that represent genuinely different formulation approaches. The five products in this guide are not a ranking or an exhaustive list. They are a focused group intended to make the differences among single-strain, multi-strain, bacterial, yeast-based, and more complex formulas easier to understand.

Probiotics are not interchangeable. Effects can depend on the strain or combination, the amount used, the outcome being studied, and the person taking it. A formula examined in one context should not automatically be recommended for another. [5]

Rather than naming one product for everyone, we focus on the information that can help you compare options and recognize where the evidence has limits.

Possibly. A probiotic does not need to become a permanent member of the gut microbiome to have an effect while it is being used.

At the same time, temporary activity should not be described as permanently restoring, resetting, or rebuilding the microbiome. Individual colonization and response vary, and a personal response cannot be predicted from the label alone. [10]

There is no universal probiotic timeline. Studies use different strains, formulas, durations, and outcome measures, so the timeline reported for one product may not apply to another.

If you are considering a probiotic for a specific concern, look at research involving the exact strain or formulation. New or persistent symptoms should not automatically be treated as a normal “adjustment period.”

Probiotics have a history of apparently safe use, particularly among healthy people, but detailed safety information remains limited. NCCIH notes that the risk of harmful effects is greater for people with severe illnesses or compromised immune systems. [14]

If you have a serious underlying condition, are pregnant or nursing, or are considering a probiotic for a child, a healthcare professional can help evaluate the exact product and situation.

There is no single interaction rule that applies to every probiotic. Bacterial and yeast organisms respond differently to antimicrobial medications, and the appropriate timing or product choice can depend on the exact probiotic and prescribed treatment.

A pharmacist or healthcare professional can review the specific combination if you take antibiotics, antifungal medications, immunosuppressants, or a complex medication regimen. Do not change the timing of prescribed medication simply to accommodate a probiotic supplement.

The Bottom Line

Probiotic labels can give you useful information, but they cannot predict exactly how a product will affect you. Strain identity, formulation transparency, potency through expiration, storage requirements, allergens, and serving details are more useful comparison points than a large CFU number or a long organism list.

The five products in this guide represent different approaches rather than a ranking. A simpler formula may be easier to connect with strain-specific research, while a more complex blend may include more components but offer less precision about what each one contributes.

You are not looking for the most impressive number on the bottle. You are looking for a formula whose ingredients, evidence limits, and practical trade-offs you understand. If your interest involves a specific health concern, medication, or higher-risk medical context, a healthcare professional or pharmacist can help you apply that information to your situation.

References

[1] FAO/WHO. (2006). Probiotics in food: Health and nutritional properties and guidelines for evaluation. FAO Food and Nutrition Paper 85. Food and Agriculture Organization of the United Nations.
 https://agris.fao.org/search/en/records/6472496908fd68d54600a230

[2] Hill, C., Guarner, F., Reid, G., Gibson, G. R., Merenstein, D. J., Pot, B., Morelli, L., Canani, R. B., Flint, H. J., Salminen, S., Calder, P. C., & Sanders, M. E. (2014). Expert consensus document: The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nature Reviews Gastroenterology & Hepatology, 11(8), 506-514.
 https://doi.org/10.1038/nrgastro.2014.66

[3] McFarland, L. V. (2015). From yaks to yogurt: The history, development, and current use of probiotics. Clinical Infectious Diseases, 60(Suppl 2), S85-S90.
 https://doi.org/10.1093/cid/civ054

[4] Thursby, E., & Juge, N. (2017). Introduction to the human gut microbiota. Biochemical Journal, 474(11), 1823-1836.
 https://doi.org/10.1042/BCJ20160510

[5] Sanders, M. E., Merenstein, D. J., Reid, G., Gibson, G. R., & Rastall, R. A. (2019). Probiotics and prebiotics in intestinal health and disease: From biology to the clinic. Nature Reviews Gastroenterology & Hepatology, 16(10), 605-616.
 https://doi.org/10.1038/s41575-019-0173-3

[6] Williams, N. T. (2010). Probiotics. American Journal of Health-System Pharmacy, 67(6), 449-458.
 https://doi.org/10.2146/ajhp090168

[7] Ouwehand, A. C., Salminen, S., & Isolauri, E. (2002). Probiotics: An overview of beneficial effects. Antonie van Leeuwenhoek, 82(1-4), 279-289.
 https://doi.org/10.1023/A:1020620607611

[8] McFarland, L. V. (2010). Systematic review and meta-analysis of Saccharomyces boulardii in adult patients. World Journal of Gastroenterology, 16(18), 2202-2222.
 https://doi.org/10.3748/wjg.v16.i18.2202

[9] Szajewska, H., Kolodziej, M., Gieruszczak-Bialek, D., Skora, A., Ruszczynski, M., & Shamir, R. (2015). Probiotics for the prevention of antibiotic-associated diarrhea in children. Journal of Pediatric Gastroenterology and Nutrition, 60(3), 337-344.
 https://doi.org/10.1097/MPG.0000000000000628

[10] Zmora, N., Zilberman-Schapira, G., Suez, J., Mor, U., Dori-Bachash, M., Bashiardes, S., Kotler, E., Zur, M., Regev-Lehavi, D., Brik, R. B., Federici, S., Cohen, Y., Linevsky, R., Rothschild, D., Moor, A. E., Ben-Moshe, S., Harmelin, A., Itzkovitz, S., Maharshak, N., ... Segal, E. (2018). Personalized gut mucosal colonization resistance to empiric probiotics is associated with unique host and microbiome features. Cell, 174(6), 1388-1405.e21.
 https://doi.org/10.1016/j.cell.2018.08.041

[11] Ford, A. C., Quigley, E. M. M., Lacy, B. E., Lembo, A. J., Saito, Y. A., Schiller, L. R., Soffer, E. E., Spiegel, B. M. R., & Moayyedi, P. (2014). Efficacy of probiotics in irritable bowel syndrome: A systematic review and meta-analysis. American Journal of Gastroenterology, 109(10), 1547-1561.
 https://doi.org/10.1038/ajg.2014.202

[12] Whorwell, P. J., Altringer, L., Morel, J., Bond, Y., Charbonneau, D., O’Mahony, L., Kiely, B., Shanahan, F., & Quigley, E. M. M. (2006). Efficacy of an encapsulated probiotic Bifidobacterium infantis 35624 in women with irritable bowel syndrome. American Journal of Gastroenterology, 101(7), 1581–1590.
 https://doi.org/10.1111/j.1572-0241.2006.00734.x

[13] Ringel-Kulka, T., McRorie, J., & Ringel, Y. (2017). Multi-center, double-blind, randomized, placebo-controlled, parallel-group study to evaluate the benefit of the probiotic Bifidobacterium infantis 35624 in non-patients with symptoms of abdominal discomfort and bloating. American Journal of Gastroenterology, 112(1), 145–151.
 https://doi.org/10.1038/ajg.2016.511

[14] National Center for Complementary and Integrative Health. (n.d.). Probiotics: Usefulness and safety.
 https://www.nccih.nih.gov/health/probiotics-usefulness-and-safety

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